Producción Científica Profesorado

Urea as the best fibrin solubilizer in the thrombin inhibition analysis: Theoretical and experimental modeling of fibrinogen denaturation



Mendoza Huizar, Luis Humberto

2020

Emmanuel Pérez-Escalante, Alma Elizabeth Cruz-Guerrero, Giaan Arturo Álvarez-Romero, Luis Humberto Mendoza-Huizar, Juan Francisco Flores-Aguilar, Luis Guillermo González-Olivares.Urea as the best fibrin solubilizer in the thrombin inhibition analysis: Theoretical and experimental modeling of fibrinogen denaturation. Chemical Physics Letters. (2020) 137352. DOI:10.1016/j.cplett.2020.137352


Abstract


Due to worldwide thrombosis incidence, new anticoagulant drugs have been developed. Therefore, diverse techniques are used to measure this capacity. In this sense, a size-exclusion chromatography method has already proposed, which depends on the fibrin solubilization step. Thus, the work aimed to establish the influence of different fibrin solubilizers on fibrinogen denaturation through theoretical-experimental modeling. Theoretical results showed that the coupling energy for fibrinogen-TCA is lower than that obtained for fibrinogen-urea and fibrinogen-bromide coupling, while experimental results evidenced partial fibrinogen precipitation with TCA, NaBr, and urea, resulting this last the best solubilizer to measure thrombin inhibition.



Producto de Investigación




Artículos relacionados

Analysis of the experimental pressuretemperature behavior in the isotropicnematic phase transition f...

Efecto de la composición del soporte sobre las estructuras superficiales de óxidos de níquel y molib...

Theoretical and Experimental Study of Cobalt Nucleation and Growth onto Gold Substrate with Differen...

A theoretical quantum study on the distribution of electrophilic and nucleophilic active sites on Ag...

Revisión de variables de diseño y condiciones de operación en la electrocoagulación

Characterization of Main Anthocyanins Extracted from Pericarp Blue Corn by MALDI-ToF MS

Influence of the Cation Nature of the Sulphate Salt on the Electrochemical Synthesis of Sulfate-Dope...

Cobalt electrodeposition onto highly oriented pyrolytic graphite (HOPG) electrode from ammonium sulf...

THEORETICAL STUDY ON THE MODIFICATION OF ACTIVE SITES IN GOLD SURFACES MODELED AS FINITE CLUSTERS: I...

Predicting the Phase Diagram of a Liquid Crystal Using the Convex Peg Model and the Semiempirical PM...