Producción Científica Profesorado

Simultaneous determination of tetracyclines in poultry muscle by capillary zone electrophoresis



Rodríguez Ávila, José Antonio

2009

Miranda, J.M., Rodriguez, J.A., Galan-Vidal, C.A., Simultaneous determination of tetracycline in poultry muscle by capillary zone electrophoresis, Journal of Chromatography A, 2009, Vol. 216, p. 3366-3371, ISSN 0021-9673


Abstract


A capillary zone electrophoresis method with UV detection was developed for the simultaneous detection and quantification of three tetracyclines in chicken meat samples: tetracycline (TC), oxytetracycline (OTC) and doxycycline (DOC). The separation conditions were: a running buffer containing 30 mM sodium phosphate, 2 mM EDTA disodium salt and 2.5% 2-propanol, pH 12.0, a 5 s hydrodynamic injection and a 14 kV separation voltage. Two different clean-up methodologies were employed: solid-phase extraction with C18 cartridges and ion exchange with Amberlite XAD7 resin. Analytes were detected at 360 nm in less than 12 min. LODs ranged from 61 ?g kg?1 for OTC to 68 ?g kg?1 for DOC with C18 cartridges, and 81 ?g kg?1 for DOC to 89 ?g kg?1 for TC with Amberlite XAD7 resin. The recoveries for TC, OTC and DOC obtained by both methods were between 85 and 95%, and the peak area repeatability for all of the samples was below 5% in all cases. Twenty-four samples of commercial chicken drumsticks were examined with both clean-up methodologies. In nine cases (37.5%) TC was detected, in a range from 197.8 to 2564.3 ?g kg?1, and in seven cases (29.2%) OTC was detected in a range from 83.0 to 2049.3 ?g kg?1. DOC was not detected in any of the tested samples. This method would be useful for the routine monitoring of TCs residues in poultry muscle.



Producto de Investigación




Artículos relacionados

Characterization of Main Anthocyanins Extracted from Pericarp Blue Corn by MALDI-ToF MS

cis-Palladium(II) complexes of derivatives of di-(2-pyridyl)methane: Study of the influence of the b...

Determination of primary amino acids in wines by high performance liquid magneto-chromatography

Potentiometric quantification of saccharin by using a selective membrane formed by pyrrole electropo...

High-performance liquid magneto-chromatography

Influence of the Cation Nature of the Sulphate Salt on the Electrochemical Synthesis of Sulfate-Dope...

Nucleation and Growth Kinetics of Electrodeposited Sulfate-Doped Polypyrrole: Determination of the D...

Metal content evaluation and its effect on the stability of anthocyanins

Electrochemical and AFM characterization of the electropolimerization of pyrrole over a graphiteepox...

Design and Construction of Solid State Ag/AgCl Reference Electrodes Through Electrochemical Depositi...