Producción Científica Profesorado

Sequential Injection Spectrophotometric Determination of Diclofenac in Urine and Pharmaceutical Formulations



Páez Hernández, María Elena

2008

Rodriguez, J.A., Islas, G., Paez-Hernandez, M.E., E. Barrado. Sequential injection spectrophotometric determination of diclofenac in urine and pharmaceutical formulations. J. Flow Injection Anal., 25:1 (2008) 3943. Preprinted


Abstract


This paper reports a sequential injection spectrophotometric method for determining the concentration of diclofenac (DCF) in urine samples and pharmaceutical formulations. The method is based on the reaction of DCF with potassium permanganate in an alkaline medium. The influence on the analytical signal of the reactor length, the permanganate volume, the sample volume and the flow rate was investigated using a Taguchi parameter design. Under optimal conditions, the linear range of the calibration curve varied from 10.0 to 100.0 mg l-1, with a detection limit of 5.0 mg l-1. A sampling rate of 15 determinations h-1 was achieved; the relative standard deviation of analytical repeatability was less than 3.0% in all cases. The method was validated by comparing the results obtained against those provided by RP-HPLC; no significant differences were seen (p<0.05)



Producto de Investigación




Artículos relacionados

Sequential Injection Spectrophotometric Determination of Diclofenac in Urine and Pharmaceutical Form...

Development of a novel composite sensor based on doped pyrrole selective to nitrate ions

Electrochemical and AFM characterization of the electropolimerization of pyrrole over a graphiteepox...

Validation of a tubular bismuth film amperometric detector: Determination of diclofenac sodium by mu...

Construction and Evaluation of a Gold Tubular Electrode for Flow Analysis: Application to Speciation...

Evaluación del contenido de metales y su efecto en la estabilidad de antocianinas

Determination of primary amino acids in wines by high performance liquid magneto-chromatography

Magnetic solid phase extraction based on phenyl silica adsorbent for the determination of tetracycli...

Prevalence and Antimicrobial Resistance Patterns of Salmonella from Different Raw Foods in Mexico

Determination of glucose by flow injection analysis with amperometric detection at Fe(III)-(tris(3,5...